Cybertory™ Sequencing Simulator - Reaction Parameters

HIV mutants Template Set

User name:
Template:
Primer sequence:
Primer strand:
Priming site:
Primer purity:
Primer concentration (µM):
Annealing temperature:
Enzyme:
Label:
Output format:

Suggested HIV genotyping primers


These can be copied and pasted into the "primer" box above. 
Name
 
Sequence
 
Location
 
proF
 
AGCCAACAGCCCCACCAG
 
2150-2167
 
proR
 
GGGCCATCCATTCCT
 
2605-2588
 
rt1F
 
GTTAAACAATGGCCATTGACAGAAGA 
2610-2635
 
rt1R
 
CCATCCCTGTGGAAGCACATTG
 
3008-2987
 
rt2F
 
TGGAAAGGATCACCAGCAATATTCCA 
3006-3031
 
rt2R
 
CTGTATTTCTGCTATTAAGTCTTTTGATG
 
3438-3462
 
rt3F
 
AAGAAGCAGAGCTAGAACTGGCAGA
 
3439-3463
 
rt3R
 
AATGCATATTGTGAGTCTG
 
4057-4039
from U.S. Patent 6,379,957

Naming Scheme

Trace file names use this naming scheme for analysis with the Staden package.