Cybertory™ Sequencing Simulator - Reaction Parameters

HIV shotgun Template Set

User name:
Template:
Primer sequence:
Primer strand:
Priming site:
Primer purity:
Primer concentration (µM):
Annealing temperature:
Enzyme:
Label:
Output format:

Suggested universal primers


These can be copied and pasted into the "primer" box above.
Name
Sequence
M13/pUC sequencing primer:
GTAAAACGACGGCCAGT
M13/pUC reverse sequencing primer:
CAGGAAACAGCTATGAC
T7 promoter sequencing primer:
TACGACTCACTATAGGG
T3 promoter sequencing primer:
ATTAACCCTCACTAAAG

Naming Scheme

Trace file names use this naming scheme for analysis with the Staden package.