Cybertory™ Sequencing Simulator - Reaction Parameters

HIV shotgun Template Set

User name:
Template:
Primer sequence:
Primer strand:
Priming site:
Primer purity:
Primer concentration (µM):
Annealing temperature:
Enzyme:
Label:
Output format:

Suggested universal primers


These can be copied and pasted into the "primer" box above. 
Name
 
Sequence
 
M13/pUC sequencing primer:
 
GTAAAACGACGGCCAGT
 
M13/pUC reverse sequencing primer:
 
CAGGAAACAGCTATGAC
 
T7 promoter sequencing primer:
 
TACGACTCACTATAGGG
 
T3 promoter sequencing primer:
 
ATTAACCCTCACTAAAG

Naming Scheme

Trace file names use this naming scheme for analysis with the Staden package.