Cybertory Sequencing Simulator

Templates for HIV shotgun

User name:
Template:
Primer sequence:
Primer strand:
Priming site:
Primer purity:
Primer concentration (uM):
Annealing temperature:
Enzyme:
Label:

Naming Scheme

Trace file names use this naming scheme for analysis with the Staden package.

Commonly used universal primers


These can be copied and pasted into the "primer" box above.
M13/pUC sequencing primer:
GTAAAACGACGGCCAGT
M13/pUC reverse sequencing primer:
CAGGAAACAGCTATGAC
T7 promoter sequencing primer:
TACGACTCACTATAGGG
T3 promoter sequencing primer:
ATTAACCCTCACTAAAG