HIV Genotyping Primers

from U.S. Patent 6,379,957

These primers are for sequence-based genotyping of HIV isolates. Open the sequence trace generator in another browser window, and copy and paste these sequences into the primer sequence box.

Sequence reads from forward and reverse primers for each segment (pro, rt1, rt2, and rt3) are shown in this diagram taken from a Gap4 assembly of the reads against the HXB2 reference sequence. The open reading frame for the pol gene, which is processed to produce the protease and polymerase proteins, is shown by the green box.