| allele | chromosome | primers | base length | repeat length | repeats range | size range |
|---|---|---|---|---|---|---|
| D1S80 | 1 | GAAACTGGCCTCCAAACACTGCCCGCCG GTCTTGTTGGAGATGCACGTGCCCCTTGC |
146 | 16 | 14 - 42 | 370 - 818 |
| TPOX | 2 | CACTAGCACCCAGAACCGTC CCTTGTCAGCGTTTATTTGCC |
82 | 4 | 5 - 14 | 102 - 138 |
| D3S1358 | 3 | ACTGCAGTCCAATCTGGGT ATGAAATCAACAGAGGCTTG |
66 | 4 | 8 - 20 | 98 - 146 |
| FGA | 4 | GCCCCATAGGTTTTGAACTCA TGATTTGTCTGTAATTGCCAGC |
108 | 4 | ? | ? |
| D5S818 | 5 | GGGTGATTTTCCTCTTTGGT TGATTCCAATCATAGCCACA |
105 | 4 | 7 - 16 | 133 - 169 |
| CSF1PO | 5 | AACCTGAGTCTGCCAAGGACTAGC TTCCACACACCACTGGCCATCTTC |
267 | 4 | 6 - 16 | 291 - 331 |
| D7S820 | 7 | TGTCATAGTTTAGAACGAACTAACG CTGAGGTATCAAAAACTCAGAGG |
174 | 4 | ? | ? |
| D8S1179 | 8 | TTTTTGTATTTCATGTGTACATTCG CGTAGCTATAATTAGTTCATTTTCA |
137 | 4 | 7 - 19 | 165 - 213 |
| TH01 | 11 | GTGGGCTGAAAAGCTCCCGATTAT GTGATTCCCATTGGCCTGTTCCTC |
134 | 4 | 7 - 14 | 162 - 190 |
| VWA | 12 | CCCTAGTGGATGATAAGAATAATCAGTATG GGACAGATGATAAATACATAGGATGGATGG |
82 | 4 | ? | ? |
| D13S317 | 13 | ACAGAAGTCTGGGATGTGGA GCCCAAAAAGACAGACAGAA |
141 | 4 | 5 - 16 | 161 - 205 |
| D16S539 | 16 | GATCCCAAGCTCTTCCTCTT ACGTTTGTGTGTGCATCTGT |
113 | 4 | 5 - 15 | 133 - 173 |
| D18S51 | 18 | CAAACCCGACTACCAGCAAC GAGCCATGTTCATGCCACTG |
234 | 4 | 7 - 27 | 262 - 342 |
| D21S11 | 21 | GTGAGTCAATTCCCCAAG GTTGTATTAGTCAATGTTCTCC |
105 | 4 | 24 - 38 | 201 - 257 |
| Amelogenin | X/Y | CTGATGGTTGGCCTCAAGCCTGTG TAAAGAGATTCATTAACTTGACTG |
X = 977 bp Y = 788 bp |
N.A. | N.A. | N.A. |
Information on the STR loci was obtained primarily from STR Fact Sheets and this table of sex-typing primers. See this map of where the FBI CODIS core STR loci are located in the gnome.
Paper on calculating probability of sibships.
Annealing Temperature = 55, Denaturation Temperature = 98, Cycles = 25, 0.1 bogomolar each primer
