Assembly Errors

Vector and quality Clipping


Good Assembly for Round 1


No Vector Clipping


Vector clipping failed because PreGap4 could not find the vector sequence file when launched by double clicking "config.pg4" on a networked drive. Moving the PreGap4 configuration file to the local "C:" drive fixed the problem. (Thanks to Dr. George Carman, University of the Pacific).

Bad Assembly for Round 1


Short segments of vector at the beginning of the reads prevents assembly because these segments do not overlap with target sequence.