Criminal DNA Database

1. Summary

The Cybertory On-line DNA Indexing System is a database of DNA patterns from simulated convicted felons and samples from unsolved crimes. Once you have determined the patterns of alleles for various STR loci in the forensics exercise, you can search this database to see if your sample matches any known criminals, or if you can link your case to any other unsolved crimes.

Compute the number of repeats in each allele to search the on-line DNA database of known felons and samples from unsolved cases.

Please note: any resemblance of these images to persons living, dead, or otherwise is completely coincidental.

2. Primers

For each sample or felon, the database stores genotypong results for a select set of loci. We use the same loci used by the FBI: detailed information on the FBI CODIS STR primers is available at http://www.cstl.nist.gov/biotech/strbase/fbicore.htm

With the exception of the amelogenin locus used for sex typing, the CODIS loci are all contain short tandem repeats (STRs)

Primer Table

Allele

(chromosome)

primers

base length

repeat length

repeats range

size range

D1S80

(1)

5'-GAAACTGGCCTCCAAACACTGCCCGCCG-3'

5'-GTCTTGTTGGAGATGCACGTGCCCCTTGC-3'

146

16

14 - 42

370 - 818

TPOX

(2)

5'-CACTAGCACCCAGAACCGTC-3'

5'-CCTTGTCAGCGTTTATTTGCC-3'

82

4

5 - 14

102 - 138

D3S1358

(3)

5'-ACTGCAGTCCAATCTGGGT-3'

5'-ATGAAATCAACAGAGGCTTG-3'

66

4

8 - 20

98 - 146

FGA

(4)

5'-GCCCCATAGGTTTTGAACTCA-3'

5'-TGATTTGTCTGTAATTGCCAGC-3'

108

4

?

?

D5S818

(5)

5'-GGGTGATTTTCCTCTTTGGT-3'

5'-TGATTCCAATCATAGCCACA-3'

105

4

7 - 16

133 - 169

CSF1PO

(5)

5'-AACCTGAGTCTGCCAAGGACTAGC-3'

5'-TTCCACACACCACTGGCCATCTTC-3'

267

4

6 - 16

291 - 331

D7S820

(7)

5'-TGTCATAGTTTAGAACGAACTAACG-3'

5'-CTGAGGTATCAAAAACTCAGAGG-3'

174

4

?

?

D8S1179

(8)

5'-TTTTTGTATTTCATGTGTACATTCG-3'

5'-CGTAGCTATAATTAGTTCATTTTCA-3'

137

4

7 - 19

165 - 213

TH01

(11)

5'-GTGGGCTGAAAAGCTCCCGATTAT-3'

5'-GTGATTCCCATTGGCCTGTTCCTC-3'

134

4

7 - 14

162 - 190

VWA

(12)

5'-CCCTAGTGGATGATAAGAATAATCAGTATG-3'

5'-GGACAGATGATAAATACATAGGATGGATGG-3'

82

4

?

?

D13S317

(13)

5'-ACAGAAGTCTGGGATGTGGA-3'

5'-GCCCAAAAAGACAGACAGAA-3'

141

4

5 - 16

161 - 205

D16S539

(16)

5'-GATCCCAAGCTCTTCCTCTT-3'

5'-ACGTTTGTGTGTGCATCTGT-3'

113

4

5 - 15

133 - 173

D18S51

(18)

5'-CAAACCCGACTACCAGCAAC-3'

5'-GAGCCATGTTCATGCCACTG-3'

234

4

7 - 27

262 - 342

D21S11

(21)

5'-GTGAGTCAATTCCCCAAG-3'

5'-GTTGTATTAGTCAATGTTCTCC-3'

105

4

24 - 38

201 - 257

Amelogenin

(X/Y)

5'-CTGATGGTTGGCCTCAAGCCTGTG-3'

5'-TAAAGAGATTCATTAACTTGACTG-3'

X = 977 bp

Y = 788 bp

N.A.

N.A.

N.A.

3. Example Results

The gels below were obtained by running the FBI CODIS primers on Pt00-Pt09 under standard conditions (annealing temperature = 55, denaturation temperature = 98, cycles = 25)

1. amelogenin

Results from running amelogenin primers on first ten individuals

2. CSF1PO

Results from running CSF1PO primers on first ten individuals

3. D13S317

Results from running D13S317 primers on first ten individuals

4. D16S539

Results from running D16S539 primers on first ten individuals

5. D18S51

Results from running D18S51 primers on first ten individuals

6. D1S80

Results from running D1S80 primers on first ten individuals

7. D21S11

Results from running D21S11 primers on first ten individuals

8. D3S1358

Results from running D3S1358 primers on first ten individuals

9. D5S818

Results from running D5S818 primers on first ten individuals

10. D8S1179

Results from running D8S1179 primers on first ten individuals

11. More example data

Another set of canned data uses custom size markers so you can count the marker bands to determine the number of repeats (SVG viewer required).