Criminal DNA Database

1. Summary

The Cybertory On-line DNA Indexing System is a database of DNA patterns from simulated convicted felons and samples from unsolved crimes. Once you have determined the patterns of alleles for various STR loci in the forensics exercise, you can search this database to see if your sample matches any known criminals, or if you can link your case to any other unsolved crimes.

Compute the number of repeats in each allele to search the on-line DNA database of known felons and samples from unsolved cases.

Please note: any resemblance of these images to persons living, dead, or otherwise is completely coincidental.

2. Primers

For each sample or felon, the database stores genotypong results for a select set of loci. We use the same loci used by the FBI: detailed information on the FBI CODIS STR primers is available at http://www.cstl.nist.gov/biotech/strbase/fbicore.htm

With the exception of the amelogenin locus used for sex typing, the CODIS loci are all contain short tandem repeats (STRs)

Primer Table

Allele (chromosome)	primers	base length	repeat length	repeats range	size range
D1S80 (1)	5'-GAAACTGGCCTCCAAACACTGCCCGCCG-3' 5'-GTCTTGTTGGAGATGCACGTGCCCCTTGC-3'	146	16	14 - 42	370 - 818
TPOX (2)	5'-CACTAGCACCCAGAACCGTC-3' 5'-CCTTGTCAGCGTTTATTTGCC-3'	82	4	5 - 14	102 - 138
D3S1358 (3)	5'-ACTGCAGTCCAATCTGGGT-3' 5'-ATGAAATCAACAGAGGCTTG-3'	66	4	8 - 20	98 - 146
FGA (4)	5'-GCCCCATAGGTTTTGAACTCA-3' 5'-TGATTTGTCTGTAATTGCCAGC-3'	108	4	?	?
D5S818 (5)	5'-GGGTGATTTTCCTCTTTGGT-3' 5'-TGATTCCAATCATAGCCACA-3'	105	4	7 - 16	133 - 169
CSF1PO (5)	5'-AACCTGAGTCTGCCAAGGACTAGC-3' 5'-TTCCACACACCACTGGCCATCTTC-3'	267	4	6 - 16	291 - 331
D7S820 (7)	5'-TGTCATAGTTTAGAACGAACTAACG-3' 5'-CTGAGGTATCAAAAACTCAGAGG-3'	174	4	?	?
D8S1179 (8)	5'-TTTTTGTATTTCATGTGTACATTCG-3' 5'-CGTAGCTATAATTAGTTCATTTTCA-3'	137	4	7 - 19	165 - 213
TH01 (11)	5'-GTGGGCTGAAAAGCTCCCGATTAT-3' 5'-GTGATTCCCATTGGCCTGTTCCTC-3'	134	4	7 - 14	162 - 190
VWA (12)	5'-CCCTAGTGGATGATAAGAATAATCAGTATG-3' 5'-GGACAGATGATAAATACATAGGATGGATGG-3'	82	4	?	?
D13S317 (13)	5'-ACAGAAGTCTGGGATGTGGA-3' 5'-GCCCAAAAAGACAGACAGAA-3'	141	4	5 - 16	161 - 205
D16S539 (16)	5'-GATCCCAAGCTCTTCCTCTT-3' 5'-ACGTTTGTGTGTGCATCTGT-3'	113	4	5 - 15	133 - 173
D18S51 (18)	5'-CAAACCCGACTACCAGCAAC-3' 5'-GAGCCATGTTCATGCCACTG-3'	234	4	7 - 27	262 - 342
D21S11 (21)	5'-GTGAGTCAATTCCCCAAG-3' 5'-GTTGTATTAGTCAATGTTCTCC-3'	105	4	24 - 38	201 - 257
Amelogenin (X/Y)	5'-CTGATGGTTGGCCTCAAGCCTGTG-3' 5'-TAAAGAGATTCATTAACTTGACTG-3'	X = 977 bp Y = 788 bp	N.A.	N.A.	N.A.

3. Example Results

The gels below were obtained by running the FBI CODIS primers on Pt00-Pt09 under standard conditions (annealing temperature = 55, denaturation temperature = 98, cycles = 25)